FastQCFastQC Report
Fri 3 Mar 2017
2072_FR.sam

Summary

[OK]Basic Statistics

MeasureValue
Filename2072_FR.sam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577893
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA10203.1194177E-67.3739793
ATCATTA5456.204904E-56.469657492-93
CGTATCA5651.02134174E-46.24014886-87
GTATGCC19004.9003575E-96.185998494
ATCCTTG8400.00701717946.15576034
GCTTGAA13252.2467319E-56.031932494
TTCCGAT11306.862868E-45.82395364
TCTGCTT13351.614365E-45.634587394
TCTTCCG14651.0380162E-45.45480972
TGCTCTT10350.0097893675.4522341
TGCTTGA13900.00167220245.073408694
TATGCCG18751.8189894E-125.01454488-89
GTCTTCT16905.4569682E-115.007364794
Produced by FastQC (version 0.11.5)